Horseradish peroxidase assay Lab Report Example | Topics and Well Written Essays - 1500 words

Horseradish peroxidase assay - Lab Report Example For each enzyme there is a small of range of pH within which it works optimally. Enzymes have  active sites in their structures. The active site is the part of the enzyme that has the correct shape and the functional groups required to bind to the substrate (Dunford, 1999). Enzyme activity can be measured in any one of these two ways: observing the rate at which the substrate disappears during a reaction or measuring the rate at which the product is formed. Enzyme assays are used in such measurements. There are two methods that have been developed for use in measuring the amount of substrates or products in a chemical reaction: continuous and fixed-timed assays. Continuous assay make use of a spectrophotometer to measure the rates at which the substrate disappears and products form in real-time (Leskovac, 2003). To measure the peroxidase activity a change in the amount of product formed will be evaluated over time. For the breakdown of peroxide by peroxidase, the simplest molecule that can be measured is O2 gas, the product of the decomposition of peroxide. To accomplish this the real volume of O2 gas produced is measured by use of an indicator. For this experiment an indicator (pyrogallol) that shows the presence of O2 gas will be used (Dunford, 2010). 2.50 cm3, 0.35 cm3, 0.10 cm3, and 0.35 cm3 of deionized water, buffer solution (at a pH of 6.0), hydrogen peroxide, and pyrogallol respectively were pipetted into two separate cuvettes labelled Cuvette 1 and Cuvette 2. The contents of the cuvettes were then mixed well using a small glass rod. The spectrophotometer was set to 420 nm after which Cuvette 1 was placed into it. 0.1 ml of the buffer solution was added to the cuvette and then stirred using a small glass rod. The readings of the spectrophotometer were recorded every 10 seconds for 5 minutes. Cuvette 2 (blank) was placed into the spectrophotometer. 0.1 ml of

Company logistic analysis and drawbacks and recomendation Research Proposal

Company logistic analysis and drawbacks and recomendation - Research Proposal Example The company is listed on the New York stock exchange and in the doe Jones. The company enjoys good revenue. In the finical year of 2011, the company made a profit of 3.9 billion and their gross sales were up to 70.4 billion. The company’s stocks enjoy good ratings and trading in the stock exchange. The dividends paid for the stocks are good and shareholders enjoy many benefits that come with the strength of the stocks (Burritt). In the year 2010, home depot bought a portable device technology. This technology is aimed at easing the process of stocking shelves. It also allows them to keep an eye on customers no matter which part of the store they are in. this has helped a lot in improving customer service. The company is an all rounded company. This is because when customers have money, it allows them to do home improvements more often. This stocking technology has allowed home depot to make huge profits. This technology helps them in knowing which products have been lifted fro m the shelves and need replacement. Because of the positive feedback that they are receiving because of this technology, it is safe to assume that they will invest more in these types of technology. The company is also searching for new technology that will help them stock their shelves better. Factors Leading to its Success Home Depot is aiming at serving customers better. They want to improve customer’s satisfaction so that they can get more revenue in sales. Some of the measures that home depot has undertaken so as to improve their services are, Customer service – continued focus on customer service is driven by philosophies of making an emotive link with the customer. These philosophies are putting the customer first and making the business simple. Home depot has opened new call centers in Utah and Georgia simply to have a more personalized connection with the customers. All these centers have been opened in the last financial year. This has played a major role in increasing the earnings and profit margins for the company. In additions, new wits such as the new arrangement system for secondary and centralized return to seller process has more than 57% of all labor hours dedicated to customer services and by the end of the financial year of 2013 the labor hours will have reached 60%. In addition, other measures will be put in place to ensure complete customer satisfaction. This will lead to increased sales bringing the stock price to a higher price. This will put the stocks in a better position than the previous year. Product authority – focus on product expertise is enabled by selling transformation and a collection of strategies, which are aimed at delivering product novelty, variety and worth. In extension of this approach, new merchandise and importance for both our expertise and D-I-Y client in a variety of divisions are the focus of the approach. In addition, expansion of showrooms to accommodate more products especially the Elect rolux, whirlpool and Frigidaire will help in helping these brands to move and reach more consumers. This is because of the exposure that these brands will have on the open market. Controlled capital distribution, efficiency and competence – the tactic to driving productivity and effectiveness is advanced through nonstop operational upgrading, incremental supply chain welfares, disciplined money distributions and expenditure control. Also building shareholder worth through complex